Journal:

Article Title: Respiratory Syncytial Virus Induces Oxidative Stress by Modulating Antioxidant Enzymes

doi: 10.1165/rcmb.2008-0330OC

Figure Lengend Snippet: Evidence of oxidative stress in A549 cells. (A) A549 or (B) small airway epithelial (SAE) cells were infected with respiratory syncytial virus (RSV) (solid bars) and harvested at 6, 15, 24, and 48 hours after infection to measure F2-isoprostanes and GSH/GSSG ratio (C). Open bars, control. *P < 0.05 relative to uninfected cells. The figure is representative of two different experiments run in triplicate.

Article Snippet: Cell Culture and Infection of Epithelial Cells with RSV A549 cells, a human alveolar type II–like epithelial cell line (American Type Culture Collection, Manassas, VA) and small alveolar epithelial (SAE) cells (from Clonetics, San Diego, CA), normal human airway epithelial cells derived from terminal bronchioli, were grown according to the manufacturer's instructions.

Techniques: Infection, Virus, Control

Journal:

Article Title: Respiratory Syncytial Virus Induces Oxidative Stress by Modulating Antioxidant Enzymes

doi: 10.1165/rcmb.2008-0330OC

Figure Lengend Snippet: RSV infection modifies the expression of AOE in A549 cells. Total cell lysates, prepared from A549 cells uninfected or infected with RSV for 6, 15, 24, and 48 hours, were resolved on 10% SDS-PAGE, and Western blot was performed using antibodies against superoxide dismutase (SOD) 1, 2, 3, catalase, and glutathione-S-transferase (GST) proteins. Membranes were stripped and reprobed for β-actin as an internal control for protein integrity and loading. (A) Lanes 1, 3, 5, and 7: uninfected control cells for 6, 15, 24, and 48 hours after infection; lanes 2, 4, 6, and 8: RSV-infected cells for corresponding time points of infection. (B) Densitometric analysis of Western blot bands. Open bars, control; solid bars, RSV. The figure is representative of two independent experiments.

Article Snippet: Cell Culture and Infection of Epithelial Cells with RSV A549 cells, a human alveolar type II–like epithelial cell line (American Type Culture Collection, Manassas, VA) and small alveolar epithelial (SAE) cells (from Clonetics, San Diego, CA), normal human airway epithelial cells derived from terminal bronchioli, were grown according to the manufacturer's instructions.

Techniques: Infection, Expressing, SDS Page, Western Blot, Control

Journal:

Article Title: Respiratory Syncytial Virus Induces Oxidative Stress by Modulating Antioxidant Enzymes

doi: 10.1165/rcmb.2008-0330OC

Figure Lengend Snippet: RSV infection down-regulates AOE gene expression in A549 and SAE cells. (A) A549 or (B) SAE cells were infected with RSV (solid bars) and harvested to prepare total RNA at various time points after infection. SOD 1, 2, 3 and catalase gene expression was measured by Q-RT-PCR. Open bars, control. *P < 0.05 relative to uninfected cells. The figure represents three independent experiments.

Article Snippet: Cell Culture and Infection of Epithelial Cells with RSV A549 cells, a human alveolar type II–like epithelial cell line (American Type Culture Collection, Manassas, VA) and small alveolar epithelial (SAE) cells (from Clonetics, San Diego, CA), normal human airway epithelial cells derived from terminal bronchioli, were grown according to the manufacturer's instructions.

Techniques: Infection, Gene Expression, Reverse Transcription Polymerase Chain Reaction, Control

Journal:

Article Title: Respiratory Syncytial Virus Induces Oxidative Stress by Modulating Antioxidant Enzymes

doi: 10.1165/rcmb.2008-0330OC

Figure Lengend Snippet: Effect of RSV infection on AOE activities in A549 and SAE cells. Total cell lysates were prepared from uninfected (open bars) and RSV-infected (solid bars) (A) A549 cells or (B) SAE cells for 6, 15, 24, and 48 hours to measure total SOD, catalase, GPx, and GST enzyme activities. The figure is representative of two independent experiments.

Article Snippet: Cell Culture and Infection of Epithelial Cells with RSV A549 cells, a human alveolar type II–like epithelial cell line (American Type Culture Collection, Manassas, VA) and small alveolar epithelial (SAE) cells (from Clonetics, San Diego, CA), normal human airway epithelial cells derived from terminal bronchioli, were grown according to the manufacturer's instructions.

Techniques: Infection

Journal:

Article Title: Respiratory Syncytial Virus Induces Oxidative Stress by Modulating Antioxidant Enzymes

doi: 10.1165/rcmb.2008-0330OC

Figure Lengend Snippet: Effect of catalytic scavengers on RSV-induced chemokine secretion. A549 cells were infected with RSV in the absence or presence of 400 μM EUK-134 or EUK-163. Culture supernatants, from uninfected and infected cells, were assayed 24 hours later for RANTES and IL-8 production by ELISA. *P < 0.05 relative to untreated, RSV-infected cells. The figure is representative of two independent experiments run in triplicate.

Article Snippet: Cell Culture and Infection of Epithelial Cells with RSV A549 cells, a human alveolar type II–like epithelial cell line (American Type Culture Collection, Manassas, VA) and small alveolar epithelial (SAE) cells (from Clonetics, San Diego, CA), normal human airway epithelial cells derived from terminal bronchioli, were grown according to the manufacturer's instructions.

Techniques: Infection, Enzyme-linked Immunosorbent Assay

Journal:

Article Title: Respiratory Syncytial Virus Induces Oxidative Stress by Modulating Antioxidant Enzymes

doi: 10.1165/rcmb.2008-0330OC

Figure Lengend Snippet: RSV infection down-regulates Nrf2 expression. A549 cells were infected with RSV for various lengths of time and harvested to prepare nuclear extracts. (A) Nuclear amounts of Nrf2 protein were determined by Western blot. Nrf2 gene expression after RSV infection was quantified in (B) A549 cells or (C) SAE cells by real-time PCR. Open bars, control; solid bars, RSV. Data are presented as fold changes and are representative of two independent experiments. *P < 0.05 relative to uninfected cells.

Article Snippet: Cell Culture and Infection of Epithelial Cells with RSV A549 cells, a human alveolar type II–like epithelial cell line (American Type Culture Collection, Manassas, VA) and small alveolar epithelial (SAE) cells (from Clonetics, San Diego, CA), normal human airway epithelial cells derived from terminal bronchioli, were grown according to the manufacturer's instructions.

Techniques: Infection, Expressing, Western Blot, Gene Expression, Real-time Polymerase Chain Reaction, Control